The Effect of Zinc Sulfate on Oxidative Stress and Lipid Profile Parameters in Male Rabbits Fed High Cholesterol Diet
karbala journal of pharmaceutical sciences,
Volume 4, Issue 5, Pages 106-118
AbstractAtherosclerosis is a chronic oxidative inflammatory disease characterized by deposition of lipids in the artery wall and infiltration of inflammatory cells. It is initiated, in part, by the interaction of oxidized low-density lipoprotein (oxLDL) with cells of the vascular wall. Atherosclerosis is the leading cause of mortality in developed countries and it is overwhelmingly contributes to approximately half of all deaths in the Western world than any other disorder. Zinc is an essential trace element that is vital in maintaining normal physiology and cellular functions. It has a potent antioxidant and anti-inflammatory action and it is used in treatment of various diseases in different systems in the body. This study was undertaken to evaluate the effect of zinc sulfate on prevention and progression of atherosclerosis in male rabbits fed high fat diet. A 32 male rabbits were enrolled in this study, divided randomly into four groups with 8 rabbits in each one. The first group, normal control group was supplied with standard chow diet for two month. The second one, hyperlipidemia-induced group was fed additionally to the standard chow diet, with a 1% (w/w) cholesterol powder. The third and fourth group, 220 mg and 440 mg zinc sulfate-treated group, was fed as group two plus 220 mg and 440 mg zinc sulfate respectively. Blood samples were collected and used to determine the concentration serum of lipid profile parameters, serum malonodialdehyde (MDA), and serum reduced glutathione (GSH) at day 0, 30 and 60. In hyperlipidemia-induced group the serum concentration of lipid profile parameters and MDA highly significantly increased while the serum concentration of GSH was significantly decreased compared to normal control group (p<0.05). Zinc sulfate did not significantly affect lipid profile (p>0.05) but instead its dramatically improved oxidative stress parameters as it significantly lowered the MDA level and increased GSH level compared with hyperlipidemia-induced group (p<0.05). From these results, we can conclude that the dose of 220 and 440 mg day of oral zinc sulfate for 60 days has a significant antioxidant effect in male rabbits fed high cholesterol diet.
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