Study the effect of partially purified L-Aspariginase on production perforin concentration of perforin and hemolytic activity from leukemia lymphocyte culture
karbala journal of pharmaceutical sciences,
2013, Volume 4, Issue 4, Pages 103-110
AbstractThis work aimed to study the effect of L Aspariginase isolated from local Withania somnifera plant on the concentration and hemolytic activity of perforin from leukemia lymphocyte cells of patients with (acute lymphocyte leukemia ALL; chronic lymphocyte leukemia CLL, in addition to healthy subjects). Blood samples were collected from 20 leukemic cases (10 ALL and 10 CLL), and the isolation of lymphocyte than proliferation of leukemia lymphocyte culture for detection of the concentration of perforin by ELISA technique and the activity of perforin by hemolytic erythrocyte assay after and before adding of L-Aspariginase. The results, showed an elevation of concentration and hemolytic activity of perforin before adding L-Aspariginase, then decreasing levels after adding L-Aspariginase compared to control.
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