Production of polyvalent and monovalent diagnostic antibodies against Pseudomonas aeruginosa
karbala journal of pharmaceutical sciences,
2018, Volume 9, Issue 14, Pages 74-81
AbstractRecents studies focus on production and purification of antibodies for rapid diagnosis, or passive therapy for treatment bacterial, viral and cancer disease.The present study is an attempt to prepare and produce polyvalent and monovalent diagnostic antibodies against Pseudomonas aeruginosa. Immuno absorbance method was dependent for polyvalent antisera purification to get monovalent antibodies against P. aeruginosa. Animal model were rabbits for hyperimmunization to get polyvalent antibodies. Slide agglutination for preliminary rabbit serum assay against P.aeruginosa antigen. ELISA was done to confirm monovalent antibodies purity. The prepared monovalent antisera was highly sensitive (96%) and highly specific (95%) to detect P. aeruginosa.
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