ISSN: 7027-2221

Keywords : Bacteriophage

Isolation of various local Bacteriophages via Simple Methods and their Effects against Multidrug Resistance Bacterial Isolates

karbala journal of pharmaceutical sciences, 2018, Volume 9, Issue 15, Pages 113-121

Recently, dissemination of antibiotic resistant bacteria considered as a major public
health concern. Therefore, bacteriophages gained great attention as a promising
alternative therapy for resistant infectious diseases.
The current study aimed to isolate bacteriophages by simple methods and determining
their efficiency against bacteria isolated from clinical samples.
Bacteria were first isolated from clinical specimens and identified using standard
bacteriological and biochemical procedures. antibiogram of the isolated bacteria were
determined using different antibiotic discs and the results were interpreted according
to the clinical laboratory standards institute (CLSI) guidelines. Then sewage samples
were processed using two protocols to isolate specific bacteriophages. Finally,
antibacterial effect of bacteriophages was determined using Double Layer Agar(DLA)
The highest lytic activity of the isolated bacteriophages was seen using the first
protocol. However, both methods showed antibacterial effect.
In conclusion, bacteriophages could be isolated using very simple methods.ان انتشار البكتيريا المقاومة للمضادات الحيوية في الآونة الأخيرة ، يعتبر أحد الاهتمامات الرئيسية للصحة
العامة . لذلك ، اكتسبت العاثيات اهتماما كبيرا كعلاج بديل واعد للأمراضالمعدية المقاومة للمضادات.
تهدف الدراسة الحالية إلى عزل العاثيات البكتيرية بواسطة طرق بسيطة وتحديد فعاليتها ضد البكتيريا المعزولة
من العينات السريرية .
بداية , تم عزل البكتيريا من العينات السريرية وتمتشخيصها باستخدام الطرق البكتريولوجية والبيوكيميائية
النموذجية . كماتمتحديد مقاومة البكتيريا المعزولة للمضادات الحيوية باستخدام أقراص مضادات حيوية
وقدتم اخذ عينات من مياه الصرف الصحي لغرض عزل العاثيات (CLSI) مختلفة وتمتفسير النتائج وفقًا ل
. (DLA) باستخدام طرق مختلفة . وأخيرا ،تم دراسة التأثير المضاد للعاثيات المعزولة باستخدام طريقة ال
تم عزل عاثيات مختلفة لها تاثير مضاد للبكتريا المقاومة للمضادات الحيوية كما لوحظ أعلىتاثير للعاثيات
المعزولة باستخدام الطريقة الأولى. نستنتج انه يمكن عزل عاثيات بكتيرية من مياه الصرف الصحي بواسطة
طرق بسيطة .

Erectile Dysfunction in Diabetic Patients in the Holy Kerbala/Iraq in 2018

karbala journal of pharmaceutical sciences, 2018, Volume 9, Issue 15, Pages 1-10

Erectile dysfunction affects more than 50% of diabetic patients and results in miserable
couple’s life, especially for young adults. Possible predictors are proper therapy and
patient’s compliance with treatment, in addition to the warning signs and symptoms of
sensory neuron and motor deficits. Therefore, this study was conducted to determine the
prevalence of Erectile dysfunction and its predictors among diabetic patients in Kerbala.
Material and methods
The study included a convenient sample of 61 patients with diabetes mellitus type I and II.
They were chosen through a systematic sampling among patients at the diabetes mellitus
clinic at Al Hussein Teaching Hospital in Holy Kerbala /Iraq in 2018. All participants
were interviewed using standard questionnaire. Analysis of data used descriptive and
analytic tools including t-test, chi-square test, logistic regression and structural Equation
modeling through the statistical Package of social sciences and Amos and Excel software
at a significance level of


diabetes mellitus
erectile dysfunction
Autonomic changes
sensory and motor changes
glycemic control.