Keywords : Chromatography
karbala journal of pharmaceutical sciences,
Volume 4, Issue 6, Pages 164-178
In the present work the Nitrofurantoin drug is determined in its pharmaceuitical preparation using High Performance Liquid Chromatography (HPLC) technique with L1 column length 25cm and 4.6mm diameter. The mobile phase of acetonitrile ,and buffer solution pH 3.3 and ratio (30:70) for used.The flow rate was 1.0 m1 min -1 , Injection volum was 100 µl of 5ppm of (NFT) . The signal was detected at 254 nm with retention time 4.592 min in the range 5-25 ppm and detectiom limit 0.3425.
This method was succefully applied for the determinarion of Nitrofurantoin its pharmuceuitical preparation with recovery of not less than 106.8%