ISSN: 7027-2221

Keywords : Microcin

Molecular detection and production of mcrocin from Escherichia coli isolated from different clinical samples

Alaa abdul-Razzaq; Mohammed Sabri Abdul-Razzaq

karbala journal of pharmaceutical sciences, Volume 4, Issue 6, Pages 92-100

In this study, nine genes responsible for microcins production were detected, from seventeen clinical isolate of E.coli collected from 50 samples by using PCR techniques. catechol microcin was present in all isolates, and microcin H47 are present in 6 isolates ,where microcin M are found in 5 isolates. Also partial purification of microcin by ammonium sulphate and dialysis are conducted in this study, and find the relationship between microcin production and virulence factors of UPEC in patients with UTI. Where microcin consider narrow spectrum antibacterial peptide that inhibit the growth of other bacteria from related species during nutrition deficiency specially in low iron level.