ISSN: 7027-2221

Keywords : Escherichia coli

Evaluation of the effectivity of some organic extracts of Aloe vera against some Pathogeneic bacteria

Yass Kudhir Yasser alalq

karbala journal of pharmaceutical sciences, Volume 9, Issue 14, Pages 42-49

The aqueous, ethanol, chloroform , and acetone extracts of Aloe vera cortex leave were studied for their antimicrobial activity against two Gram-negative bacteria ( Escherichia coli and Klebsiella pneumonia ) and Gram-positive bacteria (Staphylococcus aureus) using agar well diffusion method. The extracts revealed different levels of antimicrobial activity against the tested bacterial pathogens. St .aureus was very sensitive to all types of extracts represent by a higher inhibition zone as (35.51mm) for acetone, (6.83 mm) for ethanol and (5.60 mm) for aqueous extract. On the other hand, chloroform extract showed lowered activity against Escherichia coli only and no activity against other bacteria were found. Used antibiotics against all bacteria compare with the best organic solvent which was acetone extract against all bacteria. It gave a better minimum inhibitory concentration (MIC) which was (12.5 mg/ml), (50 mgml),(25 mg/ml) against Escherichia coli, staphylococcus aureus and klebsiella pneumonia, respectively. The results showed that the best organic solvent for Aloe vera which revealed a higher antibacterial activity was acetone extraacts.

Molecular detection and production of mcrocin from Escherichia coli isolated from different clinical samples

Alaa abdul-Razzaq; Mohammed Sabri Abdul-Razzaq

karbala journal of pharmaceutical sciences, Volume 4, Issue 6, Pages 92-100

In this study, nine genes responsible for microcins production were detected, from seventeen clinical isolate of E.coli collected from 50 samples by using PCR techniques. catechol microcin was present in all isolates, and microcin H47 are present in 6 isolates ,where microcin M are found in 5 isolates. Also partial purification of microcin by ammonium sulphate and dialysis are conducted in this study, and find the relationship between microcin production and virulence factors of UPEC in patients with UTI. Where microcin consider narrow spectrum antibacterial peptide that inhibit the growth of other bacteria from related species during nutrition deficiency specially in low iron level.