ISSN: 7027-2221

Keywords : Pharmacokinetics study

Determination of Atenolol in Human Plasma by New HPLC Method with Fluorescence Detection for Pharmacokinetics Studies

Ahmed A. Hussein

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 238-261

Purpose: A new, sensitive, specific and precise HPLC analysis method was developed for the determination of atenolol in human plasma in order to be utilized for pharmacokinetics study. Methods: The drug was extracted from plasma by liquid-liquid extraction technique using dichloromethane: 2-propanol (75:25). Bamethan sulfate was used as internal standard (IS). Samples were analyzed on ODS-3 C18 Intertsil column (150 x 4.6 mm, 5 µm), applying triethylamine (0.5%): methanol at a ratio of 90:10v/v with a final pH of 3.5 in isocratic mode as a mobile phase at a flow rate of 1.3 ml/min to attain adequate resolution. Using a Spectra autosampler, separations were performed at room temperature and monitored at an excitation wavelength of 228 nm and an emission wavelength of 298 nm after injection a 60μl sample into the HPLC system.
Results: A peak area was obtained for atenolol and bamethan with 6.4 and 10.4min retention time, respectively. The lower limit of quantitation (LLOQ) was identifiable and reproducible at 10ng/ml with a precision of 1.754%. The intra-day and inter-day precision at 30, 400 and 700 ng /ml level was found to be 1.909%, 1.571% , 1.358% and 3.229, 1.471, 3.246 respectively, always lower than the accepted criteria limits (15%). The relative recovery% of atenolol at 30, 400, and 700ng/ml was found to be 100.733, 99.948, and 98.599 respectively. Conclusions: The analysis method was found to be sensitive, accurate, and precise for the quantification of atenolol in human plasma. It was applied successfully, for pharmacokinetics studies