ISSN: 7027-2221

Keywords : Metyldopa determination

New photometric method for the determination of methyl dopa via it is oxidation through periodate using Ayah 3Sx3-3D-solar micro FI photometer. Study &application

Issam M.A.Shakir AL-Hashimi; Mohammed Jassim Hamzaha AL-Kaffiji

karbala journal of pharmaceutical sciences, Volume 3, Issue 3, Pages 167-181

A newly photometric analytical method characterized by it is speed and sensitivity was developed for the determination of methyl dopa in pure and pharmaceutical samples via its oxidation to orange-reddish colored complex through periodate reaction in alkaline media using homemade Ayah 3Sx3-3D-solar FI photometer . The orange-reddish species was determined using super bright green light emitting Diod (LED) as a source . A 230µl was taken as a reasonable sample volume for the determination of drug in pure and pharmaceutical formulations. The optimum conditions were1.4ml/min flow rate for both of sodium periodate(5mmole.L-1) and sodium hydroxide (1mmole.L-1) while allowed time for injection was 12 seconds. The linear dynamic range for the instrument response versus methyl dopa concentration was 0.1-1.4 mmole.L-1 while the L.O.D was 0.377nM/230µl sample from the stepwise dilution for the minimum concentration of lowest concentration in the linear dynamic range of the calibration graph . The correlation coefficient (r) was 0.9975 while the percentage linearity (%r2) was %99.52 . The method was applied successfully for the determination of methyl dopa in pharmaceutical preparations . Using paired t-test it was shown that there was no significant difference between the proposed method and official method and on that basis the new method can be accepted as an alternative analytical method.