ISSN: 7027-2221

Keywords : Ciprofloxacin

Histological changes in the Lacrimal gland of Rabbits after exposure to Formalin and treatment by Ciprofloxacin

Muayad Naeem Kareem; Muna Hussain .Al-aameli; Heba Abd-Alsalam Alsalame

karbala journal of pharmaceutical sciences, Volume 9, Issue 14, Pages 93-103

The present study was designed to describe the histological changes in the lacrimal gland of adult rabbits after exposure to formalin and treated by ciprofloxacin. Lacrimal gland samples of fifteen rabbits were collected and divided into three groups, group (I) included (5) rabbits represented control group, while the rabbits in groups (II) included (5) rabbits exposed to formalin, were given two drops were instilled in the one eye/ day of formalin 10% for (7) days. Group (III) which included (5) rabbits were given two drops / day of formalin 10 % were instilled in the one eye for (7) days and in eighth day were treated with eye drops of 0.3% ciprofloxacin. The ciprofloxacin eye drops were instilled in the one eye of each rabbit for (5) days. The samples were fixed in 10 % formalin, routine histological technique was done and stained with Hematoxylin and Eosin ( H& E). The present study revealed as a result of exposure to formalin, there were hypertrophy and hyperplasia in the acinar epithelial cells, increase in the secretory activity of acinar cells, destroyed in some acini also was seen, marked dilation and congestion in the blood vessels and clear dilation in the interlobular ducts. after treatment by ciprofloxacin in group (III), the histological observation of lacrimal gland was similar to that in (I) group.

A new mode for an on-line determination of Ciprofloxacin in pure and pharmaceutical preparation using CFIA with homemade Ayah 3SBGRx3- 3D solar cell microphtometer analyzer.

Nagam S. TurkieAl-Awadie; Malik H. Alaloosh Alamri

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 140-157

A new, simple, accurate, fast and sensitive spectrophotometric method has been developed for the analysis of ciprofloxacin in pure and pharmaceutical formulations by continuous flow injection analysis. The method was based on the oxidation of the drug with Ce(IV)sulfate in acidic medium to formed a pinkish-red color species which determined using homemade Ayah 3SBGR x3-3D solar cell flow injection photometer. Optimum conditions were obtained using a high intensity green light emitted diode as a source. The linear dynamic range for the instrument response versus ciprofloxacin concentration was 0.05-12 mmol.L-1 while the L.O.D was of 0.300 µg / sample. The correlation coefficient (r) was 0.9912 while percentage linearity (r2%) was 98.25%. RSD% for the repeatability (n=8) was lower than 0.6% for the determination of ciprofloxacin, at concentration of 12 mmol.L-1. The method was applied successfully for the determination of ciprofloxacin in pharmaceutical preparation. A comparison was made between the newly developed method and the classical method (UV-Vis spectrophotometry) at wave length 551nm of analysis using the standard additions method via the use of paired t-test. It showed that there was no significant difference between the quoted value of each individual company with calculated t-value at 95% confidence interval from developed method, in addition to comparison between two methods and calculate t-value, it was noticed that there was no significant difference between two methods at 95% .