ISSN: 7027-2221

Keywords : Metronidazole


New approach for the on-line turbidimetric determination of metronidazole in pharmaceutical preparation via the use of a new homemade Ayah 6SX1-T-2D Solar-continuous flow injection analyser

Nagam S.Turkey Al-Awadie; Omar A. Yassin

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 158-171

A newly developed analytical method characterized by its speed and sensitivity for the determination of metronidazole (MZ) in pure and pharmaceutical preparation via turbidimetric measurement (0-180o) by Ayah 6SX1-T-2D Solar-CFI analyser. The method was based upon the formation of greenish yellow precipitate for ion pair compound for the reaction of phosphomolybdic acid with metronidazole in aqueous medium. Turbidity was measured via the reflection of incident light that collides on the surface precipitated particals at 0-180o. Chemical and physical parameters were investigated. Linear dynamic of metronidazole is ranged from 0.05-8 mmol.L-1,with correlation coefficient r = 0.9821 . The limit of detection (S/N= 3 )(3SB)=171.15 ng/sample from the step wise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph with RSD% lower than 0.5% for 4, 4.5 mmol.L-1 (n=5) concentration of metronidazole. The method was applied successfully for the determination of metronidazole in three pharmaceutical drugs. A comparison was made between the newly developed method analysis with the classical method (HANNA instrument for turbidity measurement) using the standard addition method via the use of t-test. It was noticed that there was no significant difference between two methods at 95 % confidence level.

Spectrophotometric determination of Metronidazole via diazotization reaction with p-Hydroxy Benzaldehyde as a coupling reagent

Khalaf Faris Alsamarrai

karbala journal of pharmaceutical sciences, Volume 2, Issue 2, Pages 48-58

A New, simple and sensitive spectrophotometric method for the determination of Metronidazole (MN) has been developed. The method is based on reduction of nitro group of MN to amine group by Zn dust and concentrated hydrochloric acid in hot ethanol under stirring, then the diazonium ion was prepared and coupled with para hydroxyl benzaldehyde (p-HB) to yield Insoluble water yellow dye indicated at 410nm. The linearity of the method was between 5.8×10-5M-52.2×10-5M, the molar absorptivity was 7131.121 L.mol-1.cm-1 and Sandell index was 0.0240µg.cm-2. The method has been successfully applied to the assay of MN in pure and pharmaceutical forms