ISSN: 7027-2221

Keywords : Rats

The Gastroprotective Effect of Proton Pump Inhibitors on Ethanol - Induced Gastric Erosion in Rats

Nuha F. Obeid; Mazin H. Ouda; Zahra F. Mahdi; BasmaM. Edan; Elaf Q.Hasan

karbala journal of pharmaceutical sciences, Volume 4, Issue 6, Pages 101-109

Peptic ulcer diseases are one of the wide spread diseases. The causes of peptic ulcer diseases are increases gastric acid secretion. Studies focusing on the harmful effect of intra-gastric administration of ethanol which results in gastric mucosal injury, characterized by mucosal edema, sub-epithelial hemorrhage and inflammatory cell infiltration. Omeprazole and pantoprazole are proton pump inhibitors used in the treatment of gastric ulcer and gastroesophageal disease that inhibit gastric acid secretion by blocking the H+/K+- adenosine triphosphate enzyme. The present study was carried out to determine the anti-ulcer activity of proton pump inhibitors ( omeprazole 10mg/kg and pantoprazole 3 mg/kg) on ethanol- induced ulcer rat model. Healthy Sprague Dawley rats with 12-14 weeks of age of either sex weighing between 150-200 gm. were used for present study. The animals were divided into three groups six animals in each .The ulcer was induced by administering ethanol 50% orally and the treated groups was drenched 10mg/kg omeprazole and pantoprazole 3mg/kg. The anti-ulcer activity of omeprazole and pantoprazole was able to protect against ulcer formation by ethanol was indicated by a decrease in ulcer index of both treated groups. From this study it can be concluded that omeprazole and pantoprazole possess anti-ulcerogenic activity. Besides , omeprazole might be better than pantoprazole in protection against ethanol- induced ulcer.

Anti-oxidant effect of silymarin against DDT-induced nephrotoxicity in rats

Ali H. Mohammed; Dawser K. Al-Khishali; Nada N. Al-Shawi

karbala journal of pharmaceutical sciences, Volume 4, Issue 4, Pages 136-144

Background: Oxidative stress is a common mechanism contributing for initiation and propagation of renal damage induced by several chemicals such as DDT. Silymarin, the dried extract of the ripe seeds of the plant Silybum marianum is found to be a powerful protective agent against toxin-induced tissue injury in many organs especially the liver by its antioxidant property; accordingly, the intended property needs to be clarified in other organ subjected to toxic chemicals.
Objective: The present study was designed to evaluate the possible protective effect of silymarin on the status of oxidative stress by measuring the levels of (MDA) and glutathione (GSH) in renal tissue in addition to assessment of the serum levels of urea and creatinine and examination of possible histological renal changes induced in rats by a toxic dose of DDT.
Methods: White albino rats were administered a single oral dose of DDT (100mg/kg) to induce renal toxicity. Silymarin was orally administered twice daily dose (500 mg/kg) for 7-days prior to DDT administration, then the animals were sacrificed 24-hours after DDT-treatment. The parameters of oxidative stress, MDA contents and GSH levels were measured in renal tissue homogenate. Blood was collected for measuring serum urea and creatinine levels, in addition to the histological examination of the kidneys.
Results: Treatment of rats with silymarin for 7-days prior to DDT administration caused a significant reduction in the contents of the lipid peroxidation end product, MDA down to (61%) with the increasing in the levels of GSH levels up to (82%) in renal tissue homogenate compared to DDT-treated animals. Furthermore, silymarin was able to counteract significantly the elevation in the levels of serum urea and creatinine by about 38% and 34%, respectively compared to DDT-treated rats. Sections of rats' kidney treated with silymarin 7 days prior to DDT administration, elicited improvement in the histopathological changes induced by DDT characterized by inhibition of cloudy swelling, inflammation and necrosis.
Conclusion: According to the results obtained from this study, it is conclude that silymarin have antioxidant property through direct and/or indirect mechanism that provide protective effects against DDT-induced nephrotoxicity, and makes it a good candidate to be tried clinically in this respect.