ISSN: 7027-2221

Keywords : Rats


The effect of olive oil on ibuprofen induced Renal toxicity in female rats.

Mohammed Talat Abbas; Rabab Murtadha Abed; Nazar Jabar Metab

karbala journal of pharmaceutical sciences, Volume 8, Issue 13, Pages 167-177

Ibuprofen is an effective, cheap, and it is one of the most commonly non-steroidal anti-inflammatory drug, which are among the most frequent prescribed medications worldwide .The aim of this study is to investigate the protective effect of olive oil against ibuprofen-induced nephrotoxicity female albino rats. In this study we used 24 female white rats and divided them into 4 equal groups. Each experimental group consisted of six animals. group1, control they were fed on diet and water without any treatment, group2, ibuprofen given at dose 40 mg/kg/day orally by gastric tube for 30 days, group3,olive oil 2 ml/kg/day (oral administration) , group4, ibuprofen at dose of 40 mg/kg/day and olive oil 2 ml/kg/day (oral administration).Treatments were administered once daily for 30 days. After 30 days, biochemical and histopathological analysis were conducted to evaluate nephrotoxicity. Serum levels of urea, creatinine, calcium, glucose, phosphorus and amylase were measured. Animals treated with ibuprofen alone showed a significant increase in serum levels of urea, creatinine and glucose and significant decrease in calcium. Treatment of rats with olive oil showed significant improvement in kidney function, presumably as a result of decreased boichemical parameters associated with ibuprofen-induced nephrotoxicity. Histopathological examination of the rats kidney confirmed these observations. Therefore olive oil may protect against ibuprofen-induced nephrotoxicity.

The effect of olive oil (Olea europaea) on ibuprofen induced hepatotoxicity in female rats

Mohammed Talat Abbass; Rabab Murtadha Abed; Nazar Jabar Metab

karbala journal of pharmaceutical sciences, Volume 8, Issue 13, Pages 178-187

Ibuprofen, a propionic acid derivative, is one of the most commonly Non-steroidal anti-inflammatory drugs, which are among the most frequently prescribed medications worldwide. The aim of this study is to investigate the protective effect of olive oil against ibuprofen-induced hepatotoxicity in female albino rats. In this study we used 24 female white rats and divided them into 4 equal groups. Each experimental group consisted of 6 animals. Group1,control they were fed on diet and water without any treatment ,group2, ibuprofen given at dose 40 mg/kg/day orally by gastric tube for 30 days, group3,olive oil 2 ml/kg/day (oral administation), group 4, ibuprofen at dose of 40 mg/kg/day and olive oil 2 ml/kg/day (oral administration).Treatments were administered once daily for 30 days. After 30 days, biochemical and histopathological analysis were conducted to evaluate hepatotoxicity. Serum levels of albumin, total proteins, and activity of AST, ALP, ALT and total bilirubin were measured. Animals treated with ibuprofen alone showed a significant increase in serum levels of ALT, AST and ALP and significant decrease in albumin and total proteins. Treatment of rats with olive oil showed significant improvement in hepatic function , presumably as a result of decreased boichemical parameters associated with ibuprofen-induced hepatotoxicity. Histopathological examination of the rats liver confirmed these observations. Therefore olive oil may protect against ibuprofen-induced hepatotoxicity.

Effect of Olea Europea ( Olive oil ) on gentamicin induced hepatorenal toxicity in male rats

MOHAMMED TALAT ABBAS; ALI JELEEL ALI; IBRAHIM S.ABBAS; NEZAR METAB; HASSENEIN NOOR HADI; KASIM SAKRAN ABBAS; NEJDET ALI; Akram Abd AL- Hussein; Ayat Burhan; Tuka Hassan; Hiba Jalal

karbala journal of pharmaceutical sciences, Volume 6, Issue 10, Pages 0-0

Gentamicin is aminoglycoside antibiotic commonly used for the treatment of
Gram-negative bacterial infection. In many cases, it has been the only effective
therapeutic drug against bacterial strains resistant to other antibiotics, but nephrotoxicity
and hepatotoxicity side effects limit its use. The aim of this study is to investigate the
protective effect of olive oil against gentamicin-induced hepatorenal toxicity in male
albino rats. In this study we used 24 wistar-albino rats and divided them into 4 equal
groups. Each experimental group consisted of six animals. group1,control they were
given normal saline only ,group2,gentamicin 100 mg/kg/day intraperitoneal
(IP),group3,olive oil 5 ml/kg/day (oral administation) ,group 4,gentamicin 100 mg/kg/day
intraperitoneal (IP) and olive oil 5 ml/kg/day (oral administration).Treatments were
administered once daily for 21 days. After 21 days, biochemical and histopathological
analysis were conducted to evaluate hepatoranal toxicity. Serum levels of urea,
creatinine, cholesterol,triglyceride,and activity of AST and ALT were measured.Animals
treated with gentamicin alone showed a significant increase in serum levels of these
markers.Treatment of rats with olive oil showed significant improvement in renal and
hepatic function , presumably as a result of decreased boichemical parameters associated
with gentamicin-induced hepatorenal toxicity.Histopathological examination of the rats
kidneys and liver confirmed these observations. Therefore olive oil may protect against
gentamicin-induced hepatorenal toxicity

Protective Effect of Camel Milk Against Aspirin Induced Oxidative Stress in Male Albino Rats

MOHAMMED TALAT ABBAS

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 227-237

The aim of this study is to evaluate the protective effect of camil milk on aspirin-induced oxidative stress in the liver of male albino rats. The current study included 24 male rats, which were divided into 4 groups, each group included 6 rats: group 1 as a control group, Group 2 was given aspirin at 100 mg/kg /day, orally via gavage ,Group 3 was given camil milk orally via gavage at 1 mL/kg/day and group 4 were given aspirin at 100 mg/kg/day and (after 3 hours) camil milk at 1 mL/kg/day, orally via gavage for 30 days. The results show significantly increase in serum aspartate aminotransferase and alanine aminotransferase activity, which is associated with histopathological damage of the liver. Aspirin increased oxidative stress through the increase in malodialdehyde level and decrease in superoxide dismutase , catalase and glutathione peroxidase enzymes. This modulation of the biological parameter histological damage is significantly neutralized by the administration of the camil milk. From which we can conduct that administer of the camil milk reduce the toxicity and damage caused by the aspirin.

Gastroprotective activity of alpha-pinene in ethanol-induced gastric mucosal damage in rats

Al-Juhaishi; A. M

karbala journal of pharmaceutical sciences, Volume 5, Issue 8, Pages 38-46

Gastric ulcer is the most common disorder of the gastrointestinal tract, it occurs mainly in the stomach. α-pinene is a bicyclic terpene and its oil extracted from Lamiaceae, conifers, citrus and other plants. It has broad pharmacological activities. This study was done to assess the anti-ulcer activity of α-pinene against gastric ulcer in rat. This study included 6 groups with 6 rats in each group. Group 1 is healthy group and group 2 is control group (untreated ulcer group). Group 3 (positive group) was orally pretreated with 20 mg/kg ranitidine. Groups 4, 5, and 6 (experimental groups) were orally pre-treated with α-pinene at 500, 1000 and 1500 mg/kg doses, respectively. Result of this study appeared that the ulcer control group showed severe mucosal injury, while pre-treatment with α-pinene resulted in significantly (P<0.05) protection of gastric mucosal injury, good neutralizing properties, and increase in mucus production in comparison to ranitidine pretreated and ulcer control groups. In conclusions, the present results suggest that α-pinene exhibit an anti-ulcer activity against ethanol-induced gastric ulcer in rats.