ISSN: 7027-2221

Keywords : antibodies

Detection of specific antibodies of Escherichia coli O157:H7 isolated from children by using Eliza test

Afaf Abdul-Rahman Yousif; Dawmy; Faten A. A. Al

karbala journal of pharmaceutical sciences, Volume 6, Issue 9, Pages 70-77

This study comprises two parts: part one was conducted to isolate E.coli O157: H7 from
children in Pediatric Hospital of Karballa city. The bacteria were identified by bacteriological,
biochemical and serological methods.( 14 )isolates of E.coli O157:H7 isolated from children
with diarrhea (230) and children had urinary tract infection(200) were isolated.
Part two of the study was included detection of antibodies against E.coli O157:H7 somatic
(inactivated bacterium by heating) and flagellar (inactivated bacterium by formaldehyde)
antigens in rabbits by using ELISA test. Twelve adult healthy rabbits were divided randomly
for three equal groups A, B and C used for immunization study. All rabbits had negative fecal
bacteriological culture of E.coli O157:H7 and serum was collected before immunization.
Serum antibodies titers were estimated by using ELISA and tube agglutination test pre and
post each immunization. No antibody titers were detected Pre immunization in all rabbits.
Elisa test revealed that the group (A) showed a significant variation between the value of
antibody titers in the 2nd week and 4th week post immunization. Also in group (B) there was
a significant variations between the antibody titers in the 2th week and 4th week post
immunization. Group (A) revealed low significant (p ≤ 0.05) antibody titers in serum
(0.37±0.02) in comparison with group(B) which exhibited antibody titers in serum
(0.44±0.04). No antibody titers was detected in rabbits of the control group (C) during the
same weeks intervals. Antibody titers were estimated by tube agglutination test in immunized
groups revealed significant (p ≤ 0.05) antibody titers in serum (440±120) in group A in
comparison with group-B which exhibited antibody titers in serum (560±80).Our study
concluded the importance of detection Escherichia coli O157:H7 in children and that enzymelinked
immunosorbent posses the ability for detection antibodies against this bacteria.