ISSN: 7027-2221

Keywords : Staphylococcus aureus

Evaluation of the effectivity of some organic extracts of Aloe vera against some Pathogeneic bacteria

Yass Kudhir Yasser alalq

karbala journal of pharmaceutical sciences, Volume 9, Issue 14, Pages 42-49

The aqueous, ethanol, chloroform , and acetone extracts of Aloe vera cortex leave were studied for their antimicrobial activity against two Gram-negative bacteria ( Escherichia coli and Klebsiella pneumonia ) and Gram-positive bacteria (Staphylococcus aureus) using agar well diffusion method. The extracts revealed different levels of antimicrobial activity against the tested bacterial pathogens. St .aureus was very sensitive to all types of extracts represent by a higher inhibition zone as (35.51mm) for acetone, (6.83 mm) for ethanol and (5.60 mm) for aqueous extract. On the other hand, chloroform extract showed lowered activity against Escherichia coli only and no activity against other bacteria were found. Used antibiotics against all bacteria compare with the best organic solvent which was acetone extract against all bacteria. It gave a better minimum inhibitory concentration (MIC) which was (12.5 mg/ml), (50 mgml),(25 mg/ml) against Escherichia coli, staphylococcus aureus and klebsiella pneumonia, respectively. The results showed that the best organic solvent for Aloe vera which revealed a higher antibacterial activity was acetone extraacts.

Identification of Lipase ProducersStaphylococcus aureus and Studyingthe Effects of Some Physicochemical Factors on Lipase Production

Amir Hani Raziq; Rana A. Hanoon; Asia Fadhil Redha

karbala journal of pharmaceutical sciences, Volume 6, Issue 9, Pages 30-38

Lipases catalyze both the hydrolysis and synthesis of triacylglycerols. In addition to the
industrial uses of lipases, there is an evolving literature on their role as important microbial
virulence factors. One hundred samples were collected from different body sites and lesions of in
and out patients from both sexes who attended Al-Kadhimyia Teaching Hospital and Medical
Laboratories in Baghdad Teaching Hospital during the period from March, 2012 until
September, 2012 for the isolation and identification of Staphylococcus aureus. Identification of
bacterial isolates was performed according to the standard protocols. According to lipase activity
measurement, it was revealed that isolate no. 12 was the higher producer as compared to the
others and thus it was selected for further work.The results demonstrated that the best lipase
production was in the alkaline pH (8) and at temperature of 30oC. It is also shown that the
addition of 0.001M BaCl2 and 2mM KCl to the culture media has dramatically increased lipase
activity. Conversely, the addition of 10mM NaCl, 0.001M FeSO4.7H2O, and 2M NaF has caused
significant reduction in lipase activity.

Lipase from S. aureus, Purification and Application of Three Characterizing Experiments

Amir Hani Raziq; Asia Fadhil Redha; Rana A. Hanoon

karbala journal of pharmaceutical sciences, Volume 6, Issue 9, Pages 6-15

Many lipases have been extensively purified and characterized in terms of their activity and
stability profiles relative to pH, temperature, and their effects as metal ions and chelating agents.
One hundred samples were collected from different body sites and lesions of in and out patients
who attented Al-Kadhumiyah and Baghdad Teaching Hospitals. Identification of bacterial
isolates was performed by following the procedures mentioned elsewhere in the literature.
Purification steps of lipase included: extraction of the enzyme, precipitation of the enzyme by
ammonium sulphate, dialysis, ion-exchange chromatography, and gel filtration chromatography.
The results revealed that the enzyme was purified with a yield of 0.3 and a fold of purification of
153.7. Also, the optimum temperature and pH for lipase stability were 40 0C and pH 8,
respectively, while the molecular weight of the enzyme was 110000 daltons.