ISSN: 7027-2221

Keywords : STZ


The antihyperglycemic and antihyperlipidemic effect of ethanolic extract of Apium Graveolens (celery) in Streptozocin/ high fat diet induced hyperglycemic mice.

Alaa H. Abbas; Abdulkareem H. Abd; Shihab AbdulRhman; Ibrahim S. Abbas

karbala journal of pharmaceutical sciences, Volume 8, Issue 13, Pages 10-28

In spite of the drugs available for the treatment of diabetes and high blood lipids but still accompanied by undesirable side effects, and so a wide attention and tendency for herbal and poly herbal treatment because of the lack of side effects, and low cost and accessibility.
Celery seeds, also known scientifically as Apium graveolens belong to the plant family Apiaceae . Analysis of the active ingredients of the ethanol extract of celery seeds proved the presence of different compounds, including terpenes and fatty acids, but fatty acids were the largest proportion of the rest of the substances that were examined through GC-MS.
The aim of the current study is to verify the effectiveness of ethanolic extract of A. graveolens in reducing hyperglycemia and high lipids in hyperglycemic mice .
Fourty male mice were used in the current study and mice were divided into 4 equel groups. . Group-1 (control) received distilled water (50µl ) orally and standard diet. Group-2 (drug control) orally and injected with STZ (150 mg/Kg) intraperitonially and received distilled water (50µl per day) orally with HFD . Group-3 injected with STZ and received glyburide (p.o) 0.5 mg/kg per day as standard drug with HFD for 28 days . Group-4 injected with STZ and received ethanolic extract of celery seed (400 mg/kg) per day by gastric lavage as well as HFD.
After the treatment was completed, blood samples were collected for analysis and isolation of liver and pancreas tissue for the histological examination.
The ethanolic extract of A. graveolens showed a significant decreasing in the level of blood glucose, lipid profile , serum (C-peptide) , improved (GSH), and reduction in level of MDA when compared with untreated group. The presence of variety of active consistuents in A. graveolens like flavonoids , turbines and unsaturated fatty acids may be the reason for the antihyperglycemic and antihyperlipidemic effects.

Modulation of hyperglycemia and oxidative stress by Ezetimibe in Sreptozotocin induce diabetes mellitus rats

Atheer Majid Rashid Al-Juhaishi

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 42-50

Diabetes mellitus (DM) is metabolic diseases characterized by chronic hyperglycemia due to reducing in insulin secretion, insulin function, or both. Ezetimibe is a drug that lowers plasma cholesterol levels. A total of 18 male adult albino rats were used in this study. The animals randomized into 3 groups (of 6 rats each). Rats in first group were injected with citrate buffer only and used as healthy control group. While the rats in other two groups were injected with streptozotocin (STZ) at a dose of 60 mg/kg I.P. and treated as following (for 12 weeks), diabetic control group rats received no treatment. Ezetimibe treated group rats received Ezetimibe 6 mg/kg orally once daily. Every 2 weeks, blood glucose level is measured. At the end of 12th weeks, blood samples were collected to measure the blood glucose level and superoxide dismutase activity, and then the animals were sacrificed. The pancreas was removed for histopathology assessment for the degree of islets damage. In result, Ezetimibe was significantly (P<0.05) lower blood glucose levels in compare with the diabetic controls and the activity of SOD was significantly (P<0.05) elevated in rats treated with Ezetimibe compared with the levels in the healthy and diabetic control rats. Histological studies of the pancreas of diabetic control group revealed moderate pancreas islets damage (atrophy of β-cells and cytoplasm rich by inflammatory cells) in compared to rats received Ezetimibe which have showed normal pancreas appearance to mild pancreas islets damage. In conclusions, the present results suggest that Ezetimibe exhibit hypoglycemic and antioxidant activity in diabetic rats.