ISSN: 7027-2221

Keywords : Cholesterol

Genetic study of different genes in the formation and metabolism of neurosteroids and cholesterol

Jenan M. Mohsen Al Mosawi; Alaa Abdul Razzaq Al Nuaimi; Farah Riadh Zayny

karbala journal of pharmaceutical sciences, Volume 7, Issue 11, Pages 44-55

Some of the neurodegenerative diseases is Parkinson's disease and Alzheimer's
disease, which can arise due to damage to nerve cells in the elderly. This study
examines the different genes that express enzymes for the formation and metabolism
of neurosteroids and cholesterol. The purpose of the study is to investigate which
genes are expressed in the CNS, and that could affect the functions of the brain via the
formation and metabolism of neurosteroids. The study used the human cell line SHSY5
as the model for neuronal cells. By knowing which genes nerve cells express
would in future be able to study the effect of different drugs can have in the formation
of steroid metabolites that affect brain functions.
Methods used in this study, RT-PCR (reverse transcription polymerase chain reaction)
and gel electrophoresis to qualitatively examine gene expression in neuroblastoma.
Results from the study suggested that SH-SY5Y cells express some but not all genes
involved in the formation and metabolism of steroid hormones and cholesterol. PCR
experiments, showed expression of the genes for the enzymes CYP19A1 and 17-
hydroxysteroid dehydrogenase (17β-HSD) and the estrogen receptor ER-β.
One conclusion that can be drawn from the results of this study is to neuroblastoma
cell line SH-SY5Y may constitute a possible cell model for future research on
hormonal effects and the effect of various drugs on the formation of neurosteroids that
influence brain functions.
The purpose of this study is to investigate whether the SH-SY5Y cells (neuroblastoma
cells) can express genes required for the formation and metabolism of cholesterol and
steroid hormones (neurosteroids). The study also intends to investigate which genes
can participate and which is expressed in the formation of steroid metabolites that
affect brain functions.

Study Some of Biochemical Changes in Sera of Reaven's Syndrome Patient,s and it, s relationship with Diabetes Mellitus type II

Mousa; R.F

karbala journal of pharmaceutical sciences, Volume 5, Issue 8, Pages 56-63

Aim of this study to investigate the correlation of Reaven's Syndrome with the number and type of Diabetes Mellitus in both males and females diagnostic criteria in Iraqi patients /Babylon city .This study involved 85 diabetic patients(45 males and 40 females) with age (46-52 year) have a history of diabetes for 10 years and 80 healthy controls (40 males and 40 females) were randomly selected and they were examined for dyslipidaemia and Reaven's factors. Consulting the outpatient clinic at Merjan Teaching Hospital/Babylon city during May –July 2014 . For all patients anthropometric measures were obtained and fasting blood samples were taken for determination of blood glucose, lipid profile and C reactive protein ( CRP) level.Reaven's syndrome was diagnosed in all the samples under this research. The level of CRP was found to be significantly increased with increasing number of components of Reaven's syndrome(3.21 mg/dl) . While the lowest value of (0.13 mg/dl) was found in people with absent components of Reaven's syndrome (control). A significant positive correlation was observed between CRP, fast blood sugar (FBG) and triglycerides(TG)(3.21mg/dl ,120.7 mg/dl, and 189.14 mg/dl ,respectively, P<0.001) in patient with diabetic mellitus and a negative correlation with high density lipoprotein- cholesterol (HDL-cholesterol )(25.26 mg/dl, p=0.058) in the same time .