karbala journal of pharmaceutical sciences

A new, simple, accurate, fast and sensitive spectrophotometric method has been developed for the analysis of ciprofloxacin in pure and pharmaceutical formulations by continuous flow injection analysis. The method was based on the oxidation of the drug with Ce(IV)sulfate in acidic medium to formed a pinkish-red color species which determined using homemade Ayah 3SBGR x3-3D solar cell flow injection photometer. Optimum conditions were obtained using a high intensity green light emitted diode as a source. The linear dynamic range for the instrument response versus ciprofloxacin concentration was 0.05-12 mmol.L-1 while the L.O.D was of 0.300 µg / sample. The correlation coefficient (r) was 0.9912 while percentage linearity (r2%) was 98.25%. RSD% for the repeatability (n=8) was lower than 0.6% for the determination of ciprofloxacin, at concentration of 12 mmol.L-1. The method was applied successfully for the determination of ciprofloxacin in pharmaceutical preparation. A comparison was made between the newly developed method and the classical method (UV-Vis spectrophotometry) at wave length 551nm of analysis using the standard additions method via the use of paired t-test. It showed that there was no significant difference between the quoted value of each individual company with calculated t-value at 95% confidence interval from developed method, in addition to comparison between two methods and calculate t-value, it was noticed that there was no significant difference between two methods at 95% .

A newly developed analytical method characterized by its speed and sensitivity for the determination of metronidazole (MZ) in pure and pharmaceutical preparation via turbidimetric measurement (0-180o) by Ayah 6SX1-T-2D Solar-CFI analyser. The method was based upon the formation of greenish yellow precipitate for ion pair compound for the reaction of phosphomolybdic acid with metronidazole in aqueous medium. Turbidity was measured via the reflection of incident light that collides on the surface precipitated particals at 0-180o. Chemical and physical parameters were investigated. Linear dynamic of metronidazole is ranged from 0.05-8 mmol.L-1,with correlation coefficient r = 0.9821 . The limit of detection (S/N= 3 )(3SB)=171.15 ng/sample from the step wise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph with RSD% lower than 0.5% for 4, 4.5 mmol.L-1 (n=5) concentration of metronidazole. The method was applied successfully for the determination of metronidazole in three pharmaceutical drugs. A comparison was made between the newly developed method analysis with the classical method (HANNA instrument for turbidity measurement) using the standard addition method via the use of t-test. It was noticed that there was no significant difference between two methods at 95 % confidence level.