ISSN: 7027-2221

Keywords : spectrophotometric


Spectrophotometric determination of drug compounds in pure forms and in the pharmaceutical preparations

Qabas Naji Rashid

karbala journal of pharmaceutical sciences, Volume 8, Issue 12, Pages 1-14

A sensitive, rapid and economical spectrophotometric processes for estimation
of two drugs; methyldopa (MTD) and Fluvastatin Sodium (FLV), by “Sodium 1,2-
naphthoquine- 4-sulfonate (NQS)” as reagent in an alkaline interemediate. These
methods are digested on the forming of “complexes” among these drugs and the
chromogenic reagent (NQS). Orangish-brown colored complex formatted at (pH
12) and λmax. 462 nm for (MTD), and orange colored at (pH 11.7) and λmax. 466 nm
for (FLV). Beer’s Law is obeyed in a concentrations range of (4-40 μg/mL), (16-
64 μg/mL), with molar absorptivity (14.101×104 L/mol.cm), (22.7×104 L/mol.cm),
and correlation coefficient 0.9995, 0.9987, successively, The detection limit and
quantification limit were (1.03×10-1 μg/mL, 3.12×10-1 μg/mL ), and (1.36×10-1
μg/mL, 4.12×10-1 μg/mL ), successively. The suggested methods were prosperity
implement to the estimation of “these drugs” in pure forms and in their
pharmaceutical formulations.

Spectrophotometric Determination of Copper(II) using Murexide Reagent

Adnan Majeed Mohammad; Ahmed Jamaal Ibraheem; Mustafa Abdullah Dhiab

karbala journal of pharmaceutical sciences, Volume 7, Issue 11, Pages 339-347

a simple and sensitive spectrophotometric method has been developed for
determining copper (II) with Murexide and this method depends on formation of
a stable yellow greenish colored complex at pH=5 where it has a maximum
absorption at 476 nm , the optimal conditions for the formation of complex were
studied . It appeared that the complex real-time, stable complex consists for 30
days . the molecular ratio was identified by the continuous variation method and
mole ratios . the ion reactive ratio ( ion : Reagent ) were 1:2 , Lambert beer's
law obeyed in concentration range of 10 - 80 μg.ml-1 , relative standard
deviation 1.04% , Limit of detection 0.31 μg.ml-1 and molar absorptivity was
1.2x105 L.mole-1.cm-1 . By this method could determine copper (II) with high
precision , accurate and sensitive and agree on high-water samples.

Anew spectrophotometric method for determination of Famotidine drug

Abdulbari Alhelfi; Rajwan Abd Aljabar

karbala journal of pharmaceutical sciences, Volume 7, Issue 11, Pages 15-25

Anew and simple sensitive reproducible spectrophotometric method was used for
determination of famotidine drug(FA) in bulk sample and pharmaceutical formulation
. The determination depend on formed the prussian blue by reacting the drug with
iron(III) chloride to form iron(II) which reacts with potassium hexacyanoferrate(III).
The product measurable spectrophotometrically at 744 nm. Regression analysis of
Beers plot good correlation in the concentration range (1.00-6.00) μg.ml-1
No interference was observed from the usually existing additive in the
pharmaceutical formulation and the applicability of the method was examined by
analyzing tablets containing (FA).
The correlation coefficient of 0.9982, a relative standard deviation (RSD%)of
(2.07%) and the detection limit was (3.50×10-5) μg.ml-1and the sandell sensitivity was
(1.70×10⁻⁴ μg.cm). Recoveries were (98.25-100.5 )% .