ISSN: 7027-2221

Keywords : Levo


Spectrophotometric determination of Levo-dopa in pharmaceutical preparation via oxidative coupling organic reaction

Alaa . F . Hussein; Muneer . A .AL-Da; amy; Muhammad .H . AL-Fatlawy

karbala journal of pharmaceutical sciences, Volume 4, Issue 4, Pages 145-154

A simple, accurate and sensitive colorimetric method for the determination of L-Dopa in pure and pharmaceutical preparations has been established .The proposed method uses 4–aminoantipayrine (4-AAP) as a chromogenic reagent . The method is based on the oxidative coupling reaction of L-Dopa with 4-AAP in the presence of sodium hydroxide as alkaline media to form a red water soluble dye product , that has a maximum absorption at 519 nm . Linearity was observed in the range of (0.20 – 30.00) µg.ml-1, with molar absorptivity of (0.58×104 L.mol-1.cm-1) ,a sandall sensitivity of (0.36×10-4µg.cm-2) , correlation coefficient of 0.9999 , detection limit (0.12 µg.ml-1) and the relative standard deviation is better than (1.013%) . The method was applied successfully for the determination of L-Dopa in pharmaceutical preparations and the value of recovery % was found ( 99.68 %).