ISSN: 7027-2221

Keywords : Inorganic ions

Identification of Lipase ProducersStaphylococcus aureus and Studyingthe Effects of Some Physicochemical Factors on Lipase Production

Amir Hani Raziq; Rana A. Hanoon; Asia Fadhil Redha

karbala journal of pharmaceutical sciences, Volume 6, Issue 9, Pages 30-38

Lipases catalyze both the hydrolysis and synthesis of triacylglycerols. In addition to the
industrial uses of lipases, there is an evolving literature on their role as important microbial
virulence factors. One hundred samples were collected from different body sites and lesions of in
and out patients from both sexes who attended Al-Kadhimyia Teaching Hospital and Medical
Laboratories in Baghdad Teaching Hospital during the period from March, 2012 until
September, 2012 for the isolation and identification of Staphylococcus aureus. Identification of
bacterial isolates was performed according to the standard protocols. According to lipase activity
measurement, it was revealed that isolate no. 12 was the higher producer as compared to the
others and thus it was selected for further work.The results demonstrated that the best lipase
production was in the alkaline pH (8) and at temperature of 30oC. It is also shown that the
addition of 0.001M BaCl2 and 2mM KCl to the culture media has dramatically increased lipase
activity. Conversely, the addition of 10mM NaCl, 0.001M FeSO4.7H2O, and 2M NaF has caused
significant reduction in lipase activity.