ISSN: 7027-2221

Keywords : Anti

The protective effect of Trigonella foenum- graecum L. seeds extract in high fat diet-streptozotocin induced hyperglycemic mice

Marwa A. Al-Dabbagh; Abdulkareem H. Abd; Noor M. Nasser; Ibrahim S.Abbas

karbala journal of pharmaceutical sciences, Volume 8, Issue 13, Pages 122-136

Diabetes mellitus is a chronic metabolic disorder which affects carbohydrate, protein, and fat metabolism. associated with high morbidity and mortality. characterized by hyperglycemia together with biochemical alterations of glucose and lipid metabolism. Hyperglycemia and hyperlipidemia result in development of oxidative stress, systemic and local inflammation which were an critical factors for degradation of β-cells, insulin resistance and type II diabetes .Herbal medicine is one of the therapeutic strategies in the management of diabetes mellitus. It is considered to be less toxic and have fewer side effects than synthetic ones .The objective of this study was to investigate the possible protective effect of Trigonella foenum- graecum L. seeds(TFG) extract in High Fat Diet ∕ Streptozotocin -induced toxicity in mice. The seeds of TFG were defatted by soxhelt apparatus with n-hexane ,then marc was macerated with ethanol to obtain total ethanol extract. Thirty two male albino mice were fed high fat diet for two week plus single dose of streptozotocin 120mg ∕kg intraperitoneally to generate typeII diabetes model with increased adiposity. The protective and hypoglycemic activity of seeds extract against toxicity and hyperglycemia induced was evaluated in mice. Glimepiride was used as a standard treatment .FBG, serum C-peptide ,TC , Triglycerides ,HDL, LDL levels were measured at the end of 28th day of experiment then the animal sacrificed and liver and pancreas sections were prepared and stained for histological evaluation. This study was resulted in that (TFG) seed extract cause; FBG, serum lipid profile in hyperglycemic mice significantly decrease, It also increase serum C-peptide ,Glutathione and decrease Malondialdehyde levels significantly compared to untreated group; this was associated with improving histological features that impaired during STZ exposure. In conclusion, Defatted seeds extract has potential anti-hyperglycemic and hepatoprotective effect with free radical scavenging ability against STZ-induced toxicity.

Preparation and Evaluation of Dapsone Nanoparticles

Ahmed Najim Abood; Yasamin Abdulhadi Sallal

karbala journal of pharmaceutical sciences, Volume 8, Issue 13, Pages 320-335

Objective: Preparation of Dapsone nanoparticles to get optimized solubility, rate of dissolution and then more bioavailability.
Methods: Dapsone nanoparticles were prepared using solvent-antisolvent precipitation method. A Certain amount of drug was dissolved in water-miscible solvent (ethanol or methanol), then this solution was injected at certain speed (ml/min.) into water containing stabilizer. Upon injection, precipitation of Dapsone nanoparticles will occur immediately accompanied with stirring for 30 min. at 50˚C, and then the selective formula is lyophilized. Different formulas of Dapsone nanoparticles were prepared at different polymer types, drug: polymer ratios and different organic solvent types. Characterization of these formulas involve measurement of particle size, particle morphology, saturation solubility, release profile, crystallinity, physical and chemical compatibilities between the drug and involved stabilizers.
Results: Results show that the best formula of nanoparticle (F3) prepared by dissolving 16.6 mg/ml of Dapsone in ethanol, then 3ml was injected into to a 25 ml of water with PVP (polyvinyl pyrrolidone) as a stabilizer at ratio 1:4 (drug: stabilizer) then lyophilized to obtain nanoparticles. The best formula characterized by particle size around 28.5 nm, poly-dispersity (= 0.009), specific surface area (= 78.18), without physical or chemical incompatibility, and the dissolution rate was significantly higher than that of the raw Dapsone powder.

Anti-inflammatory effect of turmeric plant ( Curcuma longa L. ) rhizomes and myrrh (Commiphora myrrha L.) gums and ginkgo Ginkgo biloba L. leaves ( tablets ) extracts

Ahmed Abies Motar; Rajaa Ali Hussein; Meison Abdulbary

karbala journal of pharmaceutical sciences, Volume 8, Issue 13, Pages 59-69

Back ground: the plants importance resulting from their uses as a nutrition source and for their therapeutic effect. These plants include turmeric , myrrh and ginkgo.
Aim of study: this study was conducted to test the anti-inflammatory effectiveness of turmeric plant ( Curcuma longa L. ) rhizomes and myrrh (Commiphora myrrha L.) gums and ginkgo , Ginkgo biloba L. leaves ( tablets )extracts.
Method: plants under study were extracted by soxhlet using methanol as a solvent, and then was fractioned by water, chloroform, ethyl acetate and hexane. The extracts tested to determine the anti-inflammatory effect through testing their ability to maintain the stability of the membranes of red blood cells , prevent protein denaturation and heat induced hemolysis.
Results: the result showed the effectiveness of all the plants under study in resisting inflammation with compared to standard medicines (Aspirin 100μg / ml and Diclofenac sodium 100μg / ml).
Conclusion: It can be concluded that the presence of secondary metabolites as alkaloid, polyphenolic compounds and phenolic acid in the plants under study would be marked a good anti-inflammatory effect.