ISSN: 7027-2221

Keywords : Nephrocalcinosis

Role of Eruca sativa in prevention of induced nephrocalcinosis in rabbits

Uday Abdul-Reda Hussein

karbala journal of pharmaceutical sciences, Volume 9, Issue 14, Pages 65-73

Background: Nephrocalcinosis is a clinical pathologic condition characterized by abnormal deposition of calcium phosphate or calcium oxalate in the renal parenchyma, which can result in impaired kidney function.
Aim: This study aimed to evaluate the effect of Eruca sativa aqueous extract in prevention of nephrocalcinosis induced by a large dose of oxalic acid in rabbits.
Materials and Methods: Twenty four healthy domestic rabbits were equally allocated into three groups and were distributed into: normal group (without drug), oxalic acid group and Eruca sativa group. Both the first and second groups were received distilled water, while the third group received aqueous extract of Eruca sativa for ten consecutive days. Single high dose of oxalic acid was given to induce nephrocalcinosis in rabbits of the second and third groups (except normal group) after 2 hours from receiving of distilled water and Eruca sativa extract at the first day. Blood samples were collected from all animals for biochemical analysis. Urine analysis and histopathological examination were performed for all rabbits to verify the presence of crystals in urine and renal tissues.
Results: Eruca sativa aqueous extract produced highly significant reduction in blood urea nitrogen, serum creatinine , with significant reduction in serum Na+ , and highly significant elevation in serum Ca2+ and serum K+ levels, also it is produced highly significant reduction in the calcium oxalate crystals density in urine and renal tissues when compared with of oxalic acid group.
Conclusion: Current study found that the aqueous extract of Eruca sativa effectively prevented the formation and deposition of calcium oxalate crystals in renal tissues, thus preventing the occurrence of nephrocalcinosis.

Early Nephroprotective Effect of Alhagi graecorum Boiss in Rabbits Ahmed H. Ismael MBChB, PhD

Ahmed H. Ismael

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 262-270

Nephrocalcinosis is the deposition of calcium in the form of phosphate or oxalate within the renal tissue, which may lead to impaire the renal function. These crystals may aggregate and provide a nidus for the formation of renal stone. This study aims to evaluate the potential effect of Alhagi graecorum Boiss (Manna tree) in prevention of induced nephrocalcinosis in rabbit model. A twenty-one local domestic rabbits were used in the study. Oxalic acid was given orally to induce nephrocalcinosis in a dose of 333 mg /kg. The animals were allocated into three groups (seven in each); a negative control group received D.W, a positive control group treated with 25 mg/kg Hydrochlorothiazide, and a third group that treated with the aqueous extract of Manna tree roots in a dose of 1 gm/kg. Animals were treated two hours before induction of nephrocalcinosis by using one of the above-mentioned agents for each group. Renal function was assessed by estimating blood urea nitrogen (BUN) and serum creatinine, on three occasions: 1st day before induction, 3rd and 5th day after induction. These results were compared with that of the control group to determine how much the nephroprotective effect of the tested agent. Parameters of second and third groups that were treated with Hydrochlorothiazide and Manna tree respectively, showed a highly significant reduction (p≤0.001) in the levels of BUN and serum creatinine due to their diuretic effect in comparison with the negative control group. From the above one can conclude that Alhagi graecorum Boiss possess nephroprotective effect when used in a dose of 1 gm/kg against induced nephrocalcinosis in rabbits.