ISSN: 7027-2221

Author : Ali Mahdi Al-Atraqchi, Sahib

Study of optimum conditions for uricase enzyme production by Pseudomonas aeruginosa

Etab Abdul Ameer Al-Mosawi; Sahib Ali Mahdi Al-Atraqchi

karbala journal of pharmaceutical sciences, Volume 2, Issue 2, Pages 264-278

The ability of 15 bacterial isolates were tested to production uricase enzyme , by using the solid and submerged culture in the primary and secondary screening .
The isolate P.aeruginosa 7 was selected for it's best in production of enzyme . the optimum fermentation condition for uricase enzyme production by P. aeruginosa 7 were examined . Results showed that used 0.05% syrup dates as carbon source, and used o.3% from uric as inducer , 0.75% from KH2PO4 , 0.1% from Mgcl2 .
The other optimum condition for production of uricase enzyme were selected , the optimum pH was 6.5 and incubation period was after 24 hrs. , and the optimum temperature 37 CO .
However the shaking was at 150 rpm , and inoculum volume was 1 from culture volume .