ISSN: 7027-2221

Author : HusamAlmanseekanaa, Limes

Isolation and characterization of bone marrowmesenchymal stem cells from rat and rabbit; a modified method

Limes HusamAlmanseekanaa; Ali MansoorJasim; RaedHamzah Mohammed

karbala journal of pharmaceutical sciences, Volume 3, Issue 3, Pages 44-51

Bone marrow MSCs were obtained from New Zealand White rabbits (~3 kg, male) and also from white rat (Ratusratus) by the Guideline for Animal Care and use Committee for Teaching and Research using a modified procedure (1,2) Briefly, after the animals was sacrified with carbon monoxide cabinet, tibias and femurs were excised and the bone marrow was extracted under sterile conditions.The collected marrow was mixed and dispersed with PBS. After that the (MSCs) were separated by density-gradient centrifugation over ficoll. Viability of the separated MSCs was determined via trypan blue staining technique. Then, cells were seeded into ten tissue culture flasks containing complete culture medium suplimented with 10% fetal bovine serum (FBS) and antibiotics, incubated at 370C in a humidified atmosphere with 5% CO2. Once the colonies reached 80–90% confluence, they were ready to be detached with trypsin/EDTA and suspended in medium for continuous culture