ISSN: 7027-2221

Author : K, A.

Partial Purification of phenylalanine hydroxylase from urine of patients with chronic renal failure

Al-Salihi; F. G; A. H; Shaker; AL-Taie; A. K

karbala journal of pharmaceutical sciences, Volume 4, Issue 5, Pages 183-194

This study include purification of Phenylalanni hydroxylase ( PAH) from patients urine with chronic renal failure. The purification steps include,protein precipitation with ammonium sulphate solution. And ion exchange chromatography by using (DEAE – Cellulose A – 50) as anionic exchanger . Then two isoenzymes (I,II) were seperated through gel filtration by using (sephadex G-200) with purification folds 20.5 & 23.7 respectivily .
the research also include the kinetics studies of the two partially purified isoezymes (I&II) . The Km values were 4 mM .The two isoenzymes shown an optimum pH at 7.2, and optimum temperature at 25 Cº.