ISSN: 7027-2221

Author : Tarek Hanoon, Imad

Determination of Glimepiride in Drug Formulations by High Performance Liquid Chromatography

Imad Tarek Hanoon; Hussian Hassan Kharnoob

karbala journal of pharmaceutical sciences, Volume 4, Issue 6, Pages 196-212

غليميبرايد , أسيتونتريل , ماء , ميثانول- In the present work the Glimepiride drug is determined in its pharmaceuitical preparation using High Performance Liquid Chromatography (HPLC) technique with L1 column length 25cm and 4.6mm diameter. The mobile phase of acetonitrile ,water, methanol and sodium phosphate mono basic and ratio (60:20:10:10) for used.The flow rate was 1.5 m1 min -1 , Injection volum was 20 µl . The signal was detected at 230 nm with retention time 8.916 min in the range 10-50 ppm and detectiom limit 2.7×10-5. - This method was succefully applied for the determinarion of Glimepiride its pharmuceuitical preparation with recovery of not less than 100.23ِِ%