ISSN: 7027-2221

Author : Hashem Kadhim and, Najeh

Determination of optimum condition for Poly-β-Hydroxybutyrate Production from Bacillus spp .

Najeh Hashem Kadhim and; Noor Abd- ulamer Oda

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 183-194

The optimum cultural and ecological condition for Poly-β-Hydroxybutyrate Production from Bacillus spp. Isolate which was isolated from soil samples at holy Kerbala governorate were studied .
These condition represented by the type and the concentration of carbon , nitrogen and mineral salts source ,in addition to the incubation period temperature , shaking speed , initial pH of production medium and occulation volume . Results revealed that , using production medium containing Date syrup at 2% as a source of carbon and ammonium chlorate at 0.2 % as a source of nitrogen , accompanied with Potassium phosphate and magnesium sulphate at 0.105 % as a total concentration for mineral salts source . The pH was adjusted to 7 with 2% v/v occulation volume . The medium was inoculated by using shaking inocular with 40 ˚C at 200 rpm /min for 72 hr.