ISSN: 7027-2221

Author : Jaber Kahiosh, Haider


The effect of different doses from hydrocortisone on the liver tissue in the male rat

Douaa Adil Rabee; Haider Jaber Kahiosh; Saad Hamad Abdulatif

karbala journal of pharmaceutical sciences, Volume 5, Issue 7, Pages 58-66

The experiment was done in the Animal House of -Department of Biology-College of Education for Pure Sciences -University of Karbala from 20/12/2013- 20/2/2014 ,The study involved the use of (32) Sprague-Dawley rats which were divided into two groups, the first group included 16 rats and this last group was divided to four groups the first three groups were injected intraperitonial injection by double concentration of hydrocortisone (1.5,3, 4.5) mg / kg and volume 1 milliliter from weight of the body for 30 days and the the fourth group was injected by normal Saline 0.9% in the same volumes that used ,The second included 16 rats and this last group was divided to four groups the first three groups were injected intraperitonial injection by double concentration of hydrocortisone (1.5,3, 4.5) mg / kg and volume 1 milliliter from weight of the body for 30 days and the fourth group was injected by normal Saline 0.9% as control in the same volumes that used they were injected for (60) days.
The results of the study confirmed the existence of significant increase (P ≤ 0.01, P ≤ 0.05) in the liver enzyme when injected intraperitonial injection by double concentration of hydrocortisone (1.5,3, 4.5) mg / kg and volume 1 milliliter from weight of the body ALT for 30, 60 day was showed (50.6± 5.92, 58.25 ± 10.24) U/L, (41.50 ± 2.8, 49.25± 1.89, 51 ± 2.44) U/L Respectively When we compared with the control group (32.5± 8.58, 34.25 ± 4.5) U/L Respectively the existence of significant increase (P ≤ 0.01, P ≤ 0.05) in the liver enzyme AST for 30, 60 day (121 ± 45.5, 150.5± 21.1),( 94.7± 18.5, 136.5± 12.4, 200.7 ± 44.9) U/L Respectively when we compared with the control group ( 93.5 ± 11.38, 85.5± 7.5) U/L Respectively the cross-section of liver tissue notice Infiltration of inflammatory cells Figure: (4),(5), Necrosis of hepatocytes Figures: (3),( 4), (5), (6),(7) , Congestion of blood Figures: (4),(5),(7) and appearance of fatty vesicles inside cytoplasm Figure: (4),(7) . When we observe the sections of liver tissue of rats during the two periods (30, 60) day .