Author : Al-Khafaji,
karbala journal of pharmaceutical sciences,
Volume 5, Issue 8, Pages 11-20
Periodontitis is a chronic bacterial infection affects the gingiva, periodontium connective tissues and alveolar bone, results in alveolar bone resorption ultimately, tooth loss, indeed, it may causes various serious systemic complications like diabetes mellitus, cardiovascular disorders, rheumatoid arthritis, preeclampsia with low birth weight and orodigestive cancer mortality. Numerous bacteria are associated with periodontitis, Porphyromonas gingivalis is considered the main, foremost and strongest periodontal pathogen involved in periodontal diseases. Fimbriae that are encoded by fimA gene have been considered the main and the first virulence factor of this bacterium involved in adhesion, colonization, invasion, establishment and persistence within the host. This study was conducted from September, 2013 to July, 2014 to investigate the prevalence of P. gingivalis throughout monoplex PCR of 16S rRNA and multiplex PCR of fimA genes amplification in adult periodontitis population in kerbala city. Specific virulent clones of P. gingivalis may present in chronic and/or aggressive periodontitis. Some variations are found in the distribution of P. gingivalis fimA genotypes among periodontitis patients and the greater prevalence of fimA genotypes (II, IV) followed by (III, Ib) in adults with chronic periodontitis.