ISSN: 7027-2221

Author : Hanoon,


Determination of Glimepiride, Amlodipine and Nitrofurantoin by Reversed phase - High Performance Liquid Chromatography (RP- HPLC Technique)

H. H; Kharnoob; I. T; Hanoon

karbala journal of pharmaceutical sciences, Volume 6, Issue 9, Pages 138-144

The drugs were studied in this research are Glimepiride( GLM) is insulin independent and
glucose reduction level in blood of human body ,Amlodipine (AML)is lowering blood
pressure and Nitrofurantoin( NTF) is used to treat urinary tract infection. In spite of many
analytical techniques were used to determine these drugs. Modified HPLC was used in
present work ,this analytical technique is rapid, accurate, sensitive and applied for
determination of drug in its formulations. Analytical parameters for determination of drugs
GLM, AML and NTF by RP-HPLC at UV detector were as following ; mobile phas
acetonitrile:water:ethanolsodiumphalate(60:20:20),cetonitrile:water:triethyamine(45:45:10),
acetonitrile:buffer solution(30:70),flow rate (1.5,1.5,1.0) ml/minute, wave length
(230,245,254) nm, retention time (8.169,6.771,4.413) minutes ,stationary phase C18 column,
linear range 10-50, 2-10,5-25 μg/ml, limit of detection (2.8×10-5 ),(2.9×10-3),(9.3×10-3)
μg/ml, standard deviation (0.054,1.8439,0.0625) and the percentage of recovery
(100.06%,100.9%,100.8%) respectively.