ISSN: 7027-2221

Author : M. Mahood, Abdulbari


RP-HPLC Method for Simultaneous Estimation of Diclofenac sodium ,Chlorphenaramine malate and Paracetamol in Tablets

Abdulbari M. Mahood

karbala journal of pharmaceutical sciences, Volume 8, Issue 12, Pages 15-28

A simple, selective, accurate reverse phase high Performance Liquid Chromatographic (Rp-HPLC) method was developed and validated for the analysis of. Diclofenac sodium ,Chlorphenaramine malate and Paracetamol in tablets .Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5m , 25 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (55:45, v/v, pH 6.0) at a flow rate of 1.2 ml/min with UV detection at 262 nm. The retention time of Diclofenac sodium, Chlorpheniramine maleate and Paracetamol were 2.115, 2.567 and 3.021 min respectively. The developed method was validated in terms of accuracy, precision, linearity for three drugs was found in the range of 2-60 µg/ml , 2-40 µg/ml and 2-40 µg/ml for Diclofenac sodium , Chlorphenaramine malate and Paracetamol respectively. The limit of detection for Diclofenac sodium , Chlorphenaramine malate and Paracetamol was found to be 0.8957 µg/mL , 0.935 µg/mL and 0.4033 µg/mL respectively whereas, the limit of quantification was found to be 2.7229 µg /mL, 2.8335 µg /mL and 1.2220 µg /mL respectively. The average recovery was found to be 99.482%, 100.932% and 98.303% for Diclofenac sodium , Chlorphenaramine malate and Paracetamol respectively.
The proposed method was found to be accurate, precise and rapid for the simultaneous determination of Diclofenac sodium , Chlorphenaramine malate and Paracetamol.